
( Brand: Acquity ), ( Manufacturer Part Number: 186005226 ), ( Modified Item: No )
The 186005226 Acquity UPLC Protein BEH SEC 200 1.7 x 300 mm, 2.1 mm I.D. is a high-performance liquid chromatography (HPLC) column designed for protein separation and analysis. This column is part of the Acquity UPLC BEH SEC series, which is known for its superior separation efficiency and excellent reproducibility.
The column features a 200 pore size, 1.7 m particle size, and a 300 mm length, providing an ideal balance between resolution and analysis time. The SEC (Size Exclusion Chromatography) mode of operation allows for the separation of proteins based on their molecular size, making it a powerful tool for protein characterization and quantitation.
The Acquity UPLC BEH SEC 200 column is made of hydrophilic silica material, which is chemically bonded with hydroxyl groups to provide excellent protein retention and selectivity. The column is also equipped with a guard column to protect the analytical column and ensure consistent performance over time.
The Acquity UPLC BEH SEC 200 1.7 x 300 mm column is compatible with a wide range of mobile phases, including aqueous buffers and organic solvents. It can be used with various detection methods, such as UV-Vis, fluorescence, and mass spectrometry, making it a versatile tool for protein analysis in various applications.
In summary, the 186005226 Acquity UPLC Protein BEH SEC 200 1.7 x 300 mm is a high-performance HPLC column for protein separation and analysis. Its 200 pore size, 1.7 m particle size, and 300 mm length provide excellent separation efficiency and reproducibility, while its hydrophilic silica material ensures excellent protein retention and selectivity. This column is a valuable tool for protein characterization and quantitation in various applications, including biopharmaceuticals, proteomics, and basic research.
Product: Acquity UPLC Protein BEH SEC 2.0 x 150 mm, 1.7 mm I.D., 4.6 mm x 300 mm, 3.0 m
Pros:1. High Resolution: The Acquity UPLC Protein BEH SEC 2.0 column offers high resolution and separation efficiency, making it suitable for complex protein mixtures.
2. Fast Analysis: With a particle size of 3.0 m, the column delivers high speed and efficiency, reducing analysis time.
3. Low Bleed: The column has a low bleed rate, ensuring that the chromatography system remains clean and free from contamination, maintaining system performance over time.
4. Compatible with Various Detection Techniques: The Acquity UPLC Protein BEH SEC 2.0 column is compatible with a variety of detection techniques, including UV, fluorescence, and mass spectrometry.
5. Wide Operating Range: The column can handle a wide range of mobile phase pH values (1.8-8.0) and temperatures (0-60 C), allowing for flexible method development.
Cons:1. High Cost: The Acquity UPLC Protein BEH SEC 2.0 column is a high-performance chromatography column, which is typically more expensive than standard columns.
2. Limited Sample Load: Due to its small particle size, the column may have a limited sample load, which could limit the throughput of some analysis methods.
3. Requires Specific Conditions: The column may require specific mobile phase composition, flow rate, and temperature conditions to achieve optimal performance, which can be time-consuming and require optimization.
Conclusion:The Acquity UPLC Protein BEH SEC 2.0 column is a high-performance chromatography tool that offers fast analysis, high resolution, and low bleed, making it well-suited for complex protein mixtures. While it is more expensive than standard columns and may have a limited sample load, its versatility and compatibility with various detection techniques make it a valuable addition to any chromatography system.
Recommendation:If you require high-resolution separation of complex protein mixtures and are willing to invest in a high-performance column, the Acquity UPLC Protein BEH SEC 2.0 column is a strong recommendation. Consider your sample load requirements and budget before making a final decision.
Info: the shipping price is only for us 48 contiguous states.