
( Brand: Waters ), ( Manufacturer Part Number: 186002353 )
The Waters 186002353 Acquity UPLC BEH C18 Column is a high-performance liquid chromatography (HPLC) column designed for ultra-performance liquid chromatography (UPLC) applications. This column is equipped with a 2.1mm x 150mm dimensions, offering a compact and efficient solution for separating and analyzing complex mixtures.
The column features a C18 stationary phase, which is a versatile and widely used reversed-phase material in HPLC. The C18 stationary phase is composed of chemically bonded octadecyl silane (C18) groups, which interact with non-polar and slightly polar compounds, providing excellent separation efficiency for a variety of analytes.
The Acquity UPLC BEH C18 Column boasts a particle size of 1.7 m, which delivers high-speed separation and increased efficiency compared to larger particle sizes. This reduction in particle size results in faster analysis times, improved resolution, and increased sample throughput.
The column also has a narrow 120 pore size, which is suitable for separating small, hydrophobic molecules. The 120 pore size allows solvents to penetrate the stationary phase, facilitating the rapid dissolution and desorption of analytes, resulting in faster analysis times and increased efficiency.
The column is manufactured using Waters' highly robust Acquity UPLC BEH technology, which ensures consistent and reproducible results. The BEH stationary phase is made from a highly pure silica substrate and features a unique bonding chemistry, resulting in a stable and robust column that can withstand harsh operating conditions.
In summary, the Waters 186002353 Acquity UPLC BEH C18 Column is a high-performance HPLC column designed for UPLC applications. Its compact dimensions, C18 stationary phase, and small particle size make it an ideal choice for separating and analyzing complex mixtures, while its narrow pore size and robust manufacturing make it suitable for a wide range of HPLC applications.
1. High Efficiency: The 2.7um particle size ensures high efficiencies, allowing for faster separations and increased throughput.
2. Excellent Selectivity: The BEH C18 stationary phase provides excellent retention and separation capabilities for a wide range of analytes.
3. Small Internal Diameter: The 2.1mm internal diameter allows for minimal sample consumption, making it cost-effective for small-scale or low-concentration analyses.
4. Suitable for UPLC: The column is specifically designed for UPLC (Ultra Performance Liquid Chromatography), offering faster analysis times and higher resolution compared to traditional HPLC.
5. Reputable Brand: Waters is a well-respected brand in the chromatography industry, known for its high-quality products and exceptional customer support.
Cons:1. High Cost: Given its high performance and small internal diameter, the column may be more expensive compared to other standard HPLC columns.
2. Limited Sample Capacity: Due to the small internal diameter, the sample capacity may be limited, which could be a disadvantage for certain applications requiring larger sample volumes.
3. Special Requirements for UPLC: The column may require specific UPLC instrumentation and conditions, which might not be compatible with all HPLC systems.
Conclusion:The Waters 186002353 HPLC column offers exceptional performance for high-resolution separations in a compact format. However, the high cost and potential compatibility issues with standard HPLC systems should be carefully considered before making a purchasing decision. If your analysis requires fast separation times, high resolution, and minimal sample consumption, this column could be a valuable investment. For more cost-sensitive applications or compatibility with standard HPLC systems, it might be worth exploring alternative column options.
Used HPLC column Waters Acquity UPLC BEH C18 4.6mm x 150mm, cat no 186002353, Low number of injections bought from big Bahama company closeout. Lot con1/4botplIR, The and photos represent best efforts to convey information our opinions about a product.