1. Tubing, Valves & Fittings Lab Supplies For Sale
  2. sku: 10819354744131891
  3. PHENOMENEX 00D-4500-AN

PHENOMENEX 00D-4500-AN Uplc Column Kinetex Phenyl Hexyl Sx 2.1X100MM 1.7U

00d-4500-an Phenomenex Uplc Column Kinetex Phenyl-hexyl 2.1x100mm 1.7u Sx

( Brand: Phenomenex ), ( Manufacturer Part Number: 00D-4500-AN ), ( Country Of Origin: United States )

Review PHENOMENEX Uplc Column Kinetex Phenyl hexyl 2.1x100mm 1.7u Sx

The **Phenomenex 00D-4500-AN UPLC Column (Kinetex Phenyl-Hexyl, 2.1 x 100 mm, 1.7 m, SX)** is a high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC) column designed for advanced analytical separations with exceptional efficiency, robustness, and versatility. Engineered with Phenomenex s proprietary **Kinetex** core-shell particle technology, this column features a **2.1 mm internal diameter** and a **100 mm bed length**, optimized for high-resolution separations in both analytical and preparative applications. The **1.7 m particle size** enables rapid, high-efficiency chromatography with reduced backpressure compared to fully porous particles, making it ideal for modern UPLC systems that demand both speed and sensitivity. The **Phenyl-Hexyl** stationary phase combines the selectivity of a phenyl group with the hydrophobic interactions of a hexyl chain, providing a balanced retention mechanism that excels in separating a wide range of polar and nonpolar compounds, including aromatic hydrocarbons, steroids, fatty acids, and complex biological molecules. The **SX (Superior Xtended) variant** enhances the column s durability and chemical resistance, ensuring long-term stability across a broad pH range (1 10) and compatibility with a variety of mobile phase modifiers, including organic solvents and buffers. This column is particularly well-suited for **reverse-phase separations**, **normal-phase applications**, and **mixed-mode chromatography**, where its unique selectivity can distinguish between structurally similar compounds that may co-elute on traditional C18 or polar-embedded phases. Whether used in pharmaceutical analysis, environmental testing, food safety, or biochemical research, the **Kinetex Phenyl-Hexyl** delivers superior peak shape, reproducibility, and sensitivity, making it a reliable choice for laboratories seeking both performance and longevity in their chromatographic workflows.

### **Pros and Cons of buying a Phenomenex 00D-4500-AN UPLC Column (Kinetex Phenyl-Hexyl, 2.1 x 100 mm, 1.7 m, SX)**

#### **Pros**

1. **High Efficiency and Resolution**

The 1.7 m particle size provides superior separation efficiency compared to traditional 3 5 m columns, allowing for better resolution of complex mixtures. This is particularly useful for analyzing compounds with similar retention times or closely eluting isomers.

2. **Versatile Phase for Polar and Nonpolar Compounds**

The phenyl-hexyl stationary phase is a hybrid of a phenyl group (for - interactions with aromatic compounds) and a hexyl group (for hydrophobic interactions). This dual functionality makes it effective for separating a wide range of analytes, including:

- Polar and nonpolar small molecules

- Aromatic and aliphatic compounds

- Biologics (e.g., peptides, proteins) under certain conditions

- Chiral separations (with appropriate additives like chiral selectors)

3. **Compatibility with UHPLC Systems**

The column is designed for ultra-high-performance liquid chromatography (UPLC), enabling faster analysis times and lower solvent consumption compared to conventional HPLC. This is ideal for high-throughput applications.

4. **Durability and Longevity**

Phenomenex columns are known for their robustness and long lifespan, especially when used within recommended operating parameters (e.g., pH range, flow rate, and temperature). The SX (Silica-X) version is optimized for stability across a wider pH range (2 8), making it suitable for more applications than standard silica-based columns.

5. **Wide Solvent Compatibility**

The column can handle a variety of mobile phases, including aqueous-organic mixtures with buffers, salts, and organic modifiers (e.g., acetonitrile, methanol). This flexibility is useful for method development across different sample matrices.

6. **Backpressure Management**

While 1.7 m particles generate higher backpressure than larger particles, modern UHPLC systems are designed to handle this. The column s design minimizes excessive pressure spikes, making it practical for routine use.

7. **Reproducibility and Consistency**

Phenomenex columns are manufactured with tight quality control, ensuring batch-to-batch consistency in performance. This is critical for regulatory applications (e.g., pharmaceutical, food safety) where reproducibility is essential.

8. **Cost-Effective for High-Throughput Work**

Despite the higher upfront cost compared to conventional HPLC columns, the efficiency gains often reduce overall analysis time and solvent costs, making it cost-effective for laboratories with high sample volumes.

---

#### **Cons**

1. **Higher Initial Cost**

The column is more expensive than traditional 3 5 m HPLC columns due to the smaller particle size and advanced manufacturing. This may be a barrier for laboratories with limited budgets or low sample throughput.

2. **Requires UHPLC System**

The column s performance is optimized for UHPLC systems capable of handling higher pressures (typically >600 bar). Laboratories using conventional HPLC may not achieve the same efficiency or may need to invest in a new system.

3. **Sensitivity to pH and Temperature**

While the SX version improves pH stability, extreme pH conditions (outside 2 8) or high temperatures can degrade the column over time. Proper maintenance is required to extend its lifespan.

4. **Potential for Clogging**

Smaller particles and high efficiency can make the column more susceptible to clogging with particulate matter or high-molecular-weight contaminants. Pre-column filtering (e.g., 0.2 m filters) is recommended to prevent fouling.

5. **Method Development Challenges**

The hybrid phenyl-hexyl phase may require more trial-and-error during method development compared to dedicated phases (e.g., C18 for nonpolar compounds or HILIC for polar compounds). Optimization of mobile phase composition, gradient conditions, and additives (e.g., salts, organic modifiers) may be needed.

6. **Backpressure Limitations**

While modern UHPLC systems can handle the backpressure, older or less robust systems may struggle, leading to reduced flow rates or premature system failure. This can limit the column s utility in some laboratories.

7. **Limited Lifespan Under Abusive Conditions**

If used outside recommended parameters (e.g., high flow rates, improper cleaning, or exposure to strong acids/bases), the column s performance may degrade faster than expected. Proper care is necessary to maximize its lifespan.

8. **Not Ideal for All Applications**

While versatile, the phenyl-hexyl phase may not be the best choice for highly polar or ionic compounds without additional optimization (e.g., ion-pairing reagents). For such cases, alternative phases (e.g., HILIC, charged surfaces) might be more suitable.

---

### **Conclusion**

The **Phenomenex 00D-4500-AN UPLC column (Kinetex Phenyl-Hexyl, 2.1 x 100 mm, 1.7 m, SX)** is a high-performance tool well-suited for laboratories requiring efficient separation of a diverse range of compounds, particularly in UHPLC applications. Its hybrid phase offers flexibility for both polar and nonpolar analytes, and its durability makes it a reliable choice for routine and high-throughput work. However, its higher cost, requirement for UHPLC systems, and need for careful handling may pose challenges for some users.

### **Recommendation**

- **Buy if:**

- You have a UHPLC system and need high-resolution separations for complex mixtures (e.g., pharmaceuticals, natural products, biologics).

- Your workflow involves analyzing a mix of polar and nonpolar compounds where a single column would be advantageous.

- You prioritize long-term performance and reproducibility in regulated environments (e.g., pharmaceutical, food safety).

- Your budget allows for the initial investment, as the efficiency gains will likely reduce long-term costs.

- **Avoid if:**

- You lack a UHPLC system and cannot justify the upgrade.

- Your samples are highly polar or ionic, and you have not optimized the method with additives (e.g., ion-pairing reagents).

- Your budget is constrained, and you can achieve similar separations with a conventional HPLC column at a lower cost.

- You frequently analyze samples with high particulate matter without proper pre-filtering.

- **Alternatives to Consider:**

- For nonpolar compounds: **C18 or C8 UHPLC columns** (e.g., Kinetex C18).

- For polar compounds: **HILIC or charged-surface columns** (e.g., Kinetex HILIC).

- For chiral separations: **Chiral UHPLC columns** (e.g., Lux Amylose or Lux Cellulose).

- For budget-conscious users: **Conventional HPLC columns** (e.g., 3 5 m particle size) if UHPLC is not required.

In summary, this column is a strong choice for advanced separations but requires the right infrastructure and application context to justify its use.

Details:

Lot con4/4bx6Prime, The and photos represent best efforts to convey information our opinions about a product. Used UPLC column Phenomenex Kinetex Phenyl-Hexyl 1.7um 2.1x100mm, cat no 00D-4500-AN. Low number of injections bought from big Bahama company closeout.

part #: 00d4500 prices

  • $57.00-$97.00

specifications tubinglabsupplyu:

  • brand: Phenomenex
  • mpn: 00d-4500-an
  • country of origin: United States

shipping tubinglabsupplyu:

  • class: Standard
  • type: Calculated
  • transit: 3-7 Days
  • carrier: UPS
  • cost: $16.50
  • service: UPS Ground

payments tubinglabsupplyu:

  • other
  • credit card: Mastercard
  • wallet: Paypal

seller tubinglabsupplyu:

  • # reviews: 11908
  • rating: 100.0%
  • city: Derwood, Maryland

offer tubinglabsupplyu:

  • started: February 23, 2026
  • sold: 0
  • options: Ship-to-home
  • quantity: 1
  • availability: In Stock

general tubinglabsupplyu:

  • Healthcare, Lab Dental > Medical, Lab Dental Supplies > Other Medical, Lab Dental Supplies
  • condition: Used

returns tubinglabsupplyu:

  • policy: Returns Accepted
  • paid by: Buyer
  • method: Money back
  • time: 30 Days
country of origin: united states,
category: business & industrial > healthcare, lab dental > medical, lab dental supplies, sku: 10819354744131891,
Phenomenex 00D-4500 Is Similar To:
tubinglabsupplyu logo
2 offers $57.00–$97.00 USD
  • Used. Offer #1 priced at $57.00 + $16.50 shipping estimate = $73.50* total. +$16.50 shipping
  • Used. Offer #2 priced at $97.00 + $16.50 shipping estimate = $113.50* total. Lot gar0shfrontPhen.+$16.50 shipping

Copyright © tubinglabsupplyu.com